Human/Mouse PDGF-AA Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAA00B
Discontinued Product
DAA00B has been discontinued.
View all PDGF-AA products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (50 µL), Platelet-poor Heparin Plasma (50 µL), Saliva (25 µL)
Sensitivity
6.29 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva)
Product Summary for Human/Mouse PDGF-AA Quantikine ELISA Kit
The Quantikine Human/Mouse PDGF-AA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure PDGF-AA in human and mouse cell culture supernates, serum, plasma, and human saliva. It contains E. coli-expressed recombinant human PDGF-AA and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PDGF-AA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human and natural mouse PDGF-AA.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Mouse
Specificity
Natural and recombinant human and mouse PDGF-AA. Recombinant rat PDGF-AA shares 95.8% amino acid sequence identity with recombinant human PDGF-AA.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 24 | 24 | 24 |
| Mean (pg/mL) | 126 | 469 | 835 | 123 | 417 | 792 |
| Standard Deviation | 10.9 | 38.7 | 67.4 | 14.8 | 50.6 | 83.0 |
| CV% | 8.7 | 8.3 | 8.1 | 12.0 | 12.1 | 10.5 |
Recovery for Human/Mouse PDGF-AA Quantikine ELISA Kit
The recovery of PDGF-AA spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 80-107 |
| Platelet-poor EDTA Plasma (n=4) | 88 | 70-100 |
| Platelet-poor Heparin Plasma (n=4) | 93 | 83-108 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-AA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were tested with two reage
Scientific Data Images for Human/Mouse PDGF-AA Quantikine ELISA Kit
Human/Mouse PDGF-AA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AA
PDGF-AA (Platelet-Derived Growth Factor AA) is a widely expressed protein that forms disulfide linked homodimers or heterodimers with PDGF-B. PDGF-AA is expressed by monocytes, macrophages, fibroblasts, vascular smooth muscle cells, and vascular endothelial cells. It acts principally as a chemoattractant and inducer of protein synthesis in multiple cell types. PDGF-AA binds and signals through homodimers of the receptor tyrosine kinase PDGF R alpha which itself associates with FGF R1 and Neuropilin-1. PDGF-AA additionally signals through heterodimers of PDGF R alpha and PDGF R beta.
Long Name
Platelet-derived Growth Factor AA
Alternate Names
PDGFAA
Gene Symbol
PDGFA
Additional PDGF-AA Products
Product Documents for Human/Mouse PDGF-AA Quantikine ELISA Kit
Product Specific Notices for Human/Mouse PDGF-AA Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.