Human LDLR Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DLDLR0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
10.3 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Heparin Plasma, Urine)
Product Summary for Human LDLR Quantikine ELISA Kit
The Quantikine Human LDL R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LDL R in cell culture supernates, cell lysates, serum, heparin plasma, and urine. It contains NS0-expressed recombinant human LDL R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LDL R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LDL R.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human LDL R. This assay also recognizes recombinant human LDL R/PCSK9 complex.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 260 | 787 | 1598 | 287 | 823 | 1602 |
| Standard Deviation | 5.68 | 17.5 | 32.4 | 15.6 | 40.0 | 74.4 |
| CV% | 2.2 | 2.2 | 2.0 | 5.4 | 4.9 | 4.6 |
Recovery for Human LDLR Quantikine ELISA Kit
The recovery of human LDL R spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 92-106 |
| Urine (n=4) | 94 | 91-99 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LDL R were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human LDLR Quantikine ELISA Kit
Human LDL R ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LDLR
LDLR (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDLR binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface. Lack of LDLR expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface.
Long Name
Low Density Lipoprotein Receptor
Alternate Names
LDL R
Entrez Gene IDs
Gene Symbol
LDLR
Additional LDLR Products
Product Documents for Human LDLR Quantikine ELISA Kit
Product Specific Notices for Human LDLR Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.