Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D28B00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Urine (50 µL)
Sensitivity
1.87 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine)
Product Summary for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-28B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 90.7 | 279 | 560 | 98.6 | 282 | 532 |
| Standard Deviation | 2.80 | 10.5 | 11.9 | 7.15 | 19.9 | 36.1 |
| CV% | 3.1 | 3.8 | 2.1 | 7.3 | 7.1 | 6.8 |
Recovery for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 113 | 83-122 |
| Cell Lysis Buffer (n=4) | 96 | 87-103 |
| EDTA Plasma (n=4) | 111 | 93-124 |
| Serum (n=4) | 111 | 96-124 |
| Urine (n=4) | 84 | 75-96 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IL-28B were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
Human IL-28B/IFN-lambda 3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-28B/IFN-lambda 3
Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.
Long Name
Interleukin 28B
Alternate Names
IFN-lambda 3
Gene Symbol
IFNL3
Additional IL-28B/IFN-lambda 3 Products
Product Documents for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
Product Specific Notices for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.