Human IL-17 Quantikine QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK317
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
6 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-17 Quantikine QuicKit ELISA
The Quantikine™ QuicKit Human IL-17 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IL-17 levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IL-17 and antibodies raised against the recombinant protein. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-17.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Ten samples from apparently healthy volunteers were evaluated for the
presence of human IL-17 in this assay. All samples measured less than the lowest standard,
31.3 pg/mL. No medical histories were available for the donors used in this study.
ND=Non-Detectable
Cell Culture Supernates:
CD4+ T cells were isolated from human PBMCs using the MagCellect™ Human CD4+ T cell
Isolation Kit (R&D Systems® Catalog # MAGH102). CD4+ T cells were then seeded at 5 x 105
/mL
and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. T cells were left unstimulated in
culture media, or cultured with immobilized Human CD3 epsilon Antibody (R&D Systems,
Catalog # MAB100) coated at 1 μg/mL and 5 μg/mL soluble Human CD28 Antibody
(R&D Systems, Catalog # MAB342) for 5 days. Stimulated cells were then treated with 10 ng/mL
PMA and 500 ng/mL Ionomycin calcium salt (Tocris™, Catalog # 1704) for 24 hours. An aliquot
of cell culture supernate was removed and assayed for human IL-17.
| Condition | (pg/mL) |
| Unstimulated | ND |
| Stimulated | 244 |
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 197 | 1025 | 201 | 1090 |
| Standard Deviation | 7.09 | 56.4 | 6.41 | 55.2 |
| CV% | 3.6 | 5.5 | 3.2 | 5.1 |
Recovery for Human IL-17 Quantikine QuicKit ELISA
The recovery of human IL-17 spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 112 | 106-118 |
| EDTA Plasma (n=2) | 115 | 107-122 |
| Heparin Plasma (n=2) | 110 | 106-116 |
| Serum (n=2) | 83 | 79-86 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-17 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-17 Quantikine QuicKit ELISA
Human IL-17 Standard Curve QuicKit ELISA
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17/IL-17A
IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
Additional IL-17/IL-17A Products
Product Documents for Human IL-17 Quantikine QuicKit ELISA
Product Specific Notices for Human IL-17 Quantikine QuicKit ELISA
For research use only
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