Human IGFBP-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGB300
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.14 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IGFBP-3 Quantikine ELISA Kit
The Quantikine Human IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGFBP-3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IGFBP-3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IGFBP-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 4.13 | 12.7 | 31.1 | 4.06 | 11.8 | 28.5 |
| Standard Deviation | 0.20 | 0.64 | 0.71 | 0.22 | 0.76 | 2.27 |
| CV% | 4.8 | 5.0 | 2.3 | 5.4 | 6.4 | 8.0 |
Recovery for Human IGFBP-3 Quantikine ELISA Kit
The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 89-99 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGFBP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IGFBP-3 Quantikine ELISA Kit
Human IGFBP-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGFBP-3
IGFBP-3 (Insulin-like growth factor binding protein-3) modulates the biological activity of IGF. It circulates in a ternary complex with IGF and the acid-labile subunit (ALS). Proteolysis of IGFBP-3 by tissue plasminogen activator (tPA), a disintegrin and metalloproteases (ADAMs), and prostate specific antigen (PSA) contributes to IGFBP-3 degradation or a reduction in its affinity for IGF. The majority of soluble IGFBP-3 found in circulation is secreted from hepatic non-parenchymal cells. IGFBP-3 potentiates EGF-EGFR-mediated cell growth, modulates adipogenesis, and regulates TGF-beta signaling, DNA damage, apoptosis, autophagy, and gene transcription.
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
Additional IGFBP-3 Products
Product Documents for Human IGFBP-3 Quantikine ELISA Kit
Product Specific Notices for Human IGFBP-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.