Human Glypican 4 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY9195-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
156-10000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Glypican 4 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Glypican 4 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Glypican 4.. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, cell lysates, tissue lysates, serum, and plasma may improve their performance in this assay.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Glypican 4 DuoSet ELISA
Human Glypican 4 DuoSet Standard Curve
Kit Contents for Human Glypican 4 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4
Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: ELISA TMB Substrate (R&D Systems,
Catalog #DY999B)
Stop Solution:2N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Glypican 4
Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core. It is expressed in brain, kidney, adrenal gland, and fat tissue and binds to FGF basic. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors. It inhibits the dopaminergic differentiation of neurons. A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function. Glypican 4 is differentially expressed between adipose tissue depots. A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance. This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation.
Alternate Names
GPC4, K-glypican
Gene Symbol
GPC4
Additional Glypican 4 Products
Product Documents for Human Glypican 4 DuoSet ELISA
Product Specific Notices for Human Glypican 4 DuoSet ELISA
For research use only
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