Human Galectin-9 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGAL90
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (100 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
0.028 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human Galectin-9 Quantikine ELISA Kit
The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E.
coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Galectin-9.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Human Milk, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.34 | 3.58 | 6.13 | 1.44 | 3.43 | 6.05 |
| Standard Deviation | 0.05 | 0.12 | 0.22 | 0.08 | 0.18 | 0.32 |
| CV% | 3.7 | 3.4 | 3.6 | 5.6 | 5.2 | 5.3 |
EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.44 | 3.77 | 6.29 | 1.40 | 3.28 | 5.84 |
| Standard Deviation | 0.06 | 0.07 | 0.08 | 0.11 | 0.20 | 0.30 |
| CV% | 4.2 | 1.9 | 1.3 | 7.9 | 6.1 | 5.1 |
Recovery for Human Galectin-9 Quantikine ELISA Kit
The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 84-104 |
| EDTA Plasma (n=4) | 103 | 85-117 |
| Heparin Plasma (n=4) | 104 | 89-117 |
| Serum (n=4) | 106 | 86-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Galectin-9 Quantikine ELISA Kit
Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve
Human Galectin-9 ELISA Cell Culture Supernate/Saliva/Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-9
Galectin-9 is a secreted carbohydrate-binding protein with two lectin domains connected by a linker region. Progressive deletion within the linker region generates an isoform known as Ecalectin or UAT. Galectin-9 binds to carbohydrate moieties of IgE, preventing immune complex formation, mast cell degranulation, and asthmatic and cutaneous anaphylaxis reactions. It functions as an eosinophil chemoattractant and induces the maturation of dendritic cells which promote Th1 polarization. Galectin-9 binds to TIM-3, inhibiting Th1 cell and CD8+ T cell responses but promoting Treg differentiation and activity. Galectin-9 suppresses tumor cell metastasis by interfering with the associations between hyaluronic acid and CD44 and between VCAM-1 and Integrin alpha4 beta1. The Ecalectin isoform (UAT; urate transporter) can be expressed as an integral membrane protein and mediate the cellular efflux of urate.
Alternate Names
Ecalectin, GAL9, Galectin9, LGALS9
Gene Symbol
LGALS9
Additional Galectin-9 Products
Product Documents for Human Galectin-9 Quantikine ELISA Kit
Product Specific Notices for Human Galectin-9 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.