Human FAP QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK3715
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
9.01 pg/mL
Assay Range
54.7-3500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human FAP QuicKit ELISA
The Quantikine QuicKit Human FAP Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human FAP levels in cell culture supernates, serum, and plasma. It contains recombinant human FAP and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FAP showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural FAP.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FAP.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 283 | 1511 | 315 | 1835 |
| Standard Deviation | 14.1 | 103 | 34.4 | 84.2 |
| CV% | 5.0 | 6.8 | 10.9 | 4.6 |
Recovery for Human FAP QuicKit ELISA
The recovery of human FAP spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 94-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FAP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human FAP QuicKit ELISA
Human FAP Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a transmembrane serine protease, and a soluble and enzymatically active form of FAP known as antiplasmin-cleaving enzyme (APCE) circulates in human plasma. FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV/CD26, which is specific for N-terminal Xaa-Pro sequences. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.
Alternate Names
FAP, Seprase
Gene Symbol
FAP
Additional Fibroblast Activation Protein alpha/FAP Products
- All Products for Fibroblast Activation Protein alpha/FAP
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- Fibroblast Activation Protein alpha/FAP ELISA Kits
- Fibroblast Activation Protein alpha/FAP Lysates
- Fibroblast Activation Protein alpha/FAP Primary Antibodies
- Fibroblast Activation Protein alpha/FAP Proteins and Enzymes
- Fibroblast Activation Protein alpha/FAP Simple Plex
Product Documents for Human FAP QuicKit ELISA
Product Specific Notices for Human FAP QuicKit ELISA
For research use only
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