Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Name: Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
Brand: R&D Systems
SKU: DEPRU0B

R&D Systems, part of Bio-Techne | Catalog # DEPRU0B

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Product Datasheet / COA / SDS

Key Product Details

Assay Length:

2.5 hours

Human Erythropoietin/EPO Quantikine ELISA Kit, RUO Summary:

The Quantikine Human Epo Immunoassay is a 2.5 hour solid phase immunoassay designed to measure human Epo in serum and EDTA plasma. It contains recombinant human Epo, and antibodies raised against the recombinant protein. Natural human Epo showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Epo.

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View Full Technical Details for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Technical Data
Citations / Reviews

Product Specifications for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Epo.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess Intra-Assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess Inter-Assay Precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (mIU/mL)	10.3	40.3	117	10.4	41.9	118
Standard Deviation	0.37	1.45	1.97	0.86	2.2	5.78
CV%	3.6	3.6	1.7	8.3	5.3	4.9

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	84-98
Serum (n=4)	101	96-107

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human Epo in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

	Human Erythropoietin Quantikine ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

Item	Value
Entrez Gene IDs	2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names	ECYT5, EP, EPO, Erythropoietin, MGC138142, MVCD2, epoetin

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Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

R&D Systems, part of Bio-Techne | Catalog # DEPRU0B

Key Product Details

View all Erythropoietin/EPO ELISA Kits »

Product Specifications for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Precision

Recovery

Linearity

Scientific Data Examples for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Preparation and Storage

Background: Erythropoietin/EPO

Customer Reviews for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Product Documents for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

Product Datasheets

COA

Certificate of Analysis

SDS

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