Human ENPP-2/Autotaxin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DENP20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
0.157 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Urine, Human Milk)
Product Summary for Human ENPP-2/Autotaxin Quantikine ELISA Kit
The Quantikine Human ENPP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ENPP-2 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human ENPP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ENPP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ENPP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ENPP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Human Milk, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 6.5 | 13.8 | 28.9 | 6.8 | 14.0 | 28.9 |
| Standard Deviation | 0.18 | 0.51 | 0.74 | 0.32 | 0.55 | 0.83 |
| CV% | 2.8 | 3.7 | 2.6 | 4.7 | 3.9 | 2.9 |
Recovery for Human ENPP-2/Autotaxin Quantikine ELISA Kit
The recovery of ENPP-2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 93-109 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of ENPP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ENPP-2/Autotaxin Quantikine ELISA Kit
Human ENPP-2/Autotaxin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ENPP-2/Autotaxin
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 2
Alternate Names
ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2
Gene Symbol
ENPP2
Additional ENPP-2/Autotaxin Products
Product Documents for Human ENPP-2/Autotaxin Quantikine ELISA Kit
Product Specific Notices for Human ENPP-2/Autotaxin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.