Human CXCL6/GCP-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGC00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
8 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CXCL6/GCP-2 Quantikine ELISA Kit
The Quantikine Human GCP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human GCP-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GCP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human GCP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GCP-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human GCP-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 273 | 517 | 1078 | 263 | 472 | 965 |
| Standard Deviation | 13.7 | 30.5 | 57.7 | 20.7 | 35.5 | 65.6 |
| CV% | 5.0 | 5.9 | 5.4 | 7.9 | 7.5 | 6.8 |
Recovery for Human CXCL6/GCP-2 Quantikine ELISA Kit
The recovery of GCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 93-114 |
| EDTA Plasma (n=5) | 99 | 89-109 |
| Heparin Plasma (n=5) | 100 | 91-112 |
| Serum (n=5) | 95 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of GCP-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL6/GCP-2 Quantikine ELISA Kit
Human CXCL6/GCP-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL6/GCP-2
GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.
Additional CXCL6/GCP-2 Products
Product Documents for Human CXCL6/GCP-2 Quantikine ELISA Kit
Product Specific Notices for Human CXCL6/GCP-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.