Human Contactin-2/TAG1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY1714-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
156-10000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Contactin-2/TAG1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Contactin-2/TAG1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Contactin-2 / TAG1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Contactin-2/TAG1 DuoSet ELISA
Human Contactin-2 / TAG1 ELISA Standard Curve
Kit Contents for Human Contactin-2/TAG1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Contactin-2/TAG1
Contactin-2 (CNTN2), also known as TAX1 (human), TAG1 (rat), and Axonin-1 (chicken), is a cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily. Contactin-2 contains 6 Ig-like domains followed by 4 fibronectin type III-like repeats. The gene for human CNTN2 encodes a 1040 aa precursor protein with a 28 aa signal peptide and a 984 aa mature secreted protein that is attached to the membrane via a glycosyl-phosphatidylinositol(GPI)-anchor.
Alternate Names
Axonin-1, AXT, CNTN2, Contactin2, TAG1, TAX1
Gene Symbol
CNTN2
Additional Contactin-2/TAG1 Products
Product Documents for Human Contactin-2/TAG1 DuoSet ELISA
Product Specific Notices for Human Contactin-2/TAG1 DuoSet ELISA
For research use only
Loading...
Loading...
Loading...