Human CD69 DuoSet ELISA, 5 Plate

DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4

Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)

Stop Solution:2 N H2SO4(Catalog #DY994)

Microplates: R&D Systems (Catalog #DY990)

Plate Sealers:ELISA Plate Sealers (Catalog #DY992)