Human CD31/PECAM-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCD310
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)
Sensitivity
0.078 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human CD31/PECAM-1 Quantikine ELISA Kit
The Quantikine Human CD31/PECAM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD31 in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human CD31 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD31 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD31.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CD31.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.62 | 4.70 | 9.96 | 1.71 | 5.00 | 10.6 |
| Standard Deviation | 0.065 | 0.117 | 0.372 | 0.104 | 0.252 | 0.411 |
| CV% | 4.0 | 2.5 | 3.7 | 6.1 | 5.0 | 3.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.01 | 5.86 | 12.3 | 1.90 | 5.71 | 12.0 |
| Standard Deviation | 0.070 | 0.182 | 0.403 | 0.163 | 0.363 | 0.677 |
| CV% | 3.5 | 3.1 | 3.3 | 8.6 | 6.4 | 5.6 |
Recovery for Human CD31/PECAM-1 Quantikine ELISA Kit
The recovery of human CD31 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 96-112 |
| EDTA Plasma (n=4) | 100 | 97-106 |
| Heparin Plasma (n=4) | 101 | 94-108 |
| Serum (n=4) | 104 | 100-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human CD31 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD31/PECAM-1 Quantikine ELISA Kit
Human CD31/PECAM-1 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve
Human CD31/PECAM-1 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD31/PECAM-1
CD31/PECAM-1 is a heavily glycosylated transmembrane homophilic adhesion protein that is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and NK cells. It is required for the transepithelial migration of leukocytes. CD31 signaling increases the activation threshold of T cells and inhibits dendritic cell development. The extracellular domain of CD31 is released during endothelial cell apoptosis. This fragment circulates in the serum of patients suffering from myocardial infarction, acute ischaemic stroke, and multiple sclerosis, conditions that involve tissue damage and endothelial cell apoptosis.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for Human CD31/PECAM-1 Quantikine ELISA Kit
Product Specific Notices for Human CD31/PECAM-1 Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.