Human CD155/PVR DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2530-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
250-16000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human CD155/PVR DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human CD155/PVR DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD155. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, may improve their performance in this assay.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human CD155/PVR DuoSet ELISA
Human CD155/PVR DuoSet Standard Curve
Kit Contents for Human CD155/PVR DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008B) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4
Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: ELISA TMB Substrate (R&D Systems,
Catalog #DY999B)
Stop Solution:2N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD155/PVR
CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like 5), is a 70 kDa type I transmembrane glycoprotein that is a member of the nectin-like family. CD155 is expressed in all normal tissues and highly expressed in tumor cells of epithelial and neuronal origin. CD155 was orginally isolated based on its ability to mediate poliovirus attachment to host cells.
Long Name
Poliovirus Receptor
Alternate Names
CD155, HVED, Necl-5, PVR, PVS
Entrez Gene IDs
Gene Symbol
PVR
Additional CD155/PVR Products
Product Documents for Human CD155/PVR DuoSet ELISA
Product Specific Notices for Human CD155/PVR DuoSet ELISA
For research use only
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