Human CD117/c-kit Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSCR00
Discontinued Product
DSCR00 has been discontinued.
View all CD117/c-kit products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
0.339 ng/mL
Assay Range
1.56-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CD117/c-kit Quantikine ELISA Kit
The Quantikine Human SCF sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SCF sR in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human SCF sR and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SCF sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SCF sR.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human SCF sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 5.68 | 13.8 | 26.4 | 5.32 | 13.6 | 26.9 |
| Standard Deviation | 0.228 | 0.390 | 0.707 | 0.481 | 0.509 | 1.21 |
| CV% | 4.0 | 2.8 | 2.7 | 9.0 | 3.7 | 4.5 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 4.69 | 14.1 | 25.7 | 5.90 | 15.1 | 28.7 |
| Standard Deviation | 0.298 | 0.359 | 0.562 | 0.555 | 0.688 | 1.76 |
| CV% | 6.4 | 2.5 | 2.2 | 9.4 | 4.6 | 6.1 |
Recovery for Human CD117/c-kit Quantikine ELISA Kit
The recovery of SCF sR spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 97-110 |
| EDTA Plasma (n=4) | 91 | 84-106 |
| Heparin Plasma (n=4) | 92 | 87-102 |
| Serum (n=4) | 93 | 86-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SCF sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD117/c-kit Quantikine ELISA Kit
Human CD117/c-kit ELISA Serum/Plasma Standard Curve
Human CD117/c-kit ELISA Cell Culture Supernate Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD117/c-kit
CD117, also known as c-kit and SCF R, is the cellular receptor for Stem Cell Factor (SCF), also named c-kit ligand, mast cell growth factor, and steel factor. This receptor-ligand system plays essential roles in germ cell development, melanogenesis, hematopoiesis, and oncogenesis. CD117 is expressed on lymphoid, myeloid, erythroid, and megakaryocyte progenitors, NK cells, germ cells, melanocytes, glial cells, vascular smooth muscle cells, placenta, and epithelial cells. Altered levels and mutations in CD117 are associated with several types of cancer including lung, breast, gastrointestinal stromal, and germ cell tumors.
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for Human CD117/c-kit Quantikine ELISA Kit
Product Specific Notices for Human CD117/c-kit Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.