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Rat Ghrelin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49745

Novus Biologicals, part of Bio-Techne
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NBP3-49745

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

0.1 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Ghrelin in samples. No significant cross-reactivity or interference between Rat Ghrelin and analogues was observed

Description

Assay Length: 2 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Rat Ghrelin ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Rat Ghrelin ELISA Kit (Colorimetric)
Rat Ghrelin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Ghrelin ELISA Kit (Colorimetric)

Rat Ghrelin ELISA Kit (Colorimetric)

ELISA: Rat Ghrelin ELISA Kit (Colorimetric) [NBP3-49745]

Standard Curve Reference.

Kit Contents for Rat Ghrelin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ghrelin

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Alternate Names

GHRL, Motilin-related Peptide, Protein M46

Gene Symbol

GHRL

Additional Ghrelin Products

Product Documents for Rat Ghrelin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Ghrelin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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