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Human Frataxin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49725

Novus Biologicals, part of Bio-Techne
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NBP3-49725

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

14.06 pg/mL

Assay Range

23.44 - 1500 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Frataxin in samples. No significant cross-reactivity or interference between Human Frataxin and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Human Frataxin ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Human Frataxin ELISA Kit (Colorimetric)

Scientific Data Images for Human Frataxin ELISA Kit (Colorimetric)

Human Frataxin ELISA Kit (Colorimetric)

ELISA: Human Frataxin ELISA Kit (Colorimetric) [NBP3-49725]

Standard Curve Reference.

Kit Contents for Human Frataxin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Frataxin

Frataxin encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Alternate Names

CyaY, EC 1.16.3.1, FA, FARRmitochondrial, frataxin, Friedreich ataxia, Friedreich ataxia protein, Fxn, X25MGC57199

Gene Symbol

FXN

Additional Frataxin Products

Product Documents for Human Frataxin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Frataxin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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