Rat FoxO1/FKHR ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42504
Key Product Details
Sample Type & Volume Required Per Well
Tissue homogenates, Cell lysates and other biological fluids (100 uL)
Sensitivity
0.122 ng/mL (example only; lot dependent)
Assay Range
0.312 - 20 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Rat
Description
Assay time: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Kit Contents for Rat FoxO1/FKHR ELISA Kit (Colorimetric)
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: FoxO1/FKHR
FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.
Long Name
Forkhead Box O1
Alternate Names
FKH1, FKHR
Gene Symbol
FOXO1
Additional FoxO1/FKHR Products
Product Documents for Rat FoxO1/FKHR ELISA Kit (Colorimetric)
Product Specific Notices for Rat FoxO1/FKHR ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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