Skip to main content

Canine Folic acid ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59997

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-59997-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.195 ng/mL

Assay Range

0.195 ng/mL - 50 ng/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Canine

Specificity

This assay has high sensitivity and excellent specificity for detection of canine folic acid. No significant cross-reactivity or interference between canine folic acid and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Canine Folic acid ELISA Kit (Colorimetric)

Canine Folic acid ELISA Kit (Colorimetric)

Canine Folic acid ELISA Kit (Colorimetric) [NBP2-59997] -

Canine Folic acid ELISA Kit (Colorimetric) [NBP2-59997] - Standard Curve Reference

Kit Contents for Canine Folic acid ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody (100 x concentrate)
  • Antibody Diluent
  • Assay plate (12 x 8 coated Microwells)
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluen
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Folic acid

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for folic acid and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated folic acid and folic acid in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of folic acid in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

Folate, Tetrahydrofolate, Tetrahydrofolic acid, THFA

Additional Folic acid Products

Product Documents for Canine Folic acid ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Canine Folic acid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Loading...
Loading...
Loading...
Loading...