Human FGF-12 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31615
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)
Sensitivity
8.1 pg/mL (example only; lot dependent)
Assay Range
15.625 - 1000 pg/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
Assay Length: 4.5 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human FGF-12 ELISA Kit (Colorimetric)
ELISA: Human FGF-12 ELISA Kit (Colorimetric) [NBP3-31615] -
ELISA: Human FGF-12 ELISA Kit (Colorimetric) [NBP3-31615] - Standard Curve ReferenceKit Contents for Human FGF-12 ELISA Kit (Colorimetric)
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: FGF-12
FGF-12 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, and -4, respectively, form a subgroup within the FGF family. Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered cytoplasmic. It also possess an N-terminal bipartite nuclear localization signal at aa 11-18 and 28-38.
Long Name
Fibroblast Growth Factor 12
Alternate Names
FGF12, FHF1
Gene Symbol
FGF12
Additional FGF-12 Products
Product Documents for Human FGF-12 ELISA Kit (Colorimetric)
Product Specific Notices for Human FGF-12 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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