Human Estrone ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1908

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Plasma (EDTA) and Serum (25 uL)

Sensitivity

14.6 pg/mL

Assay Range

8.1 to 2400 pg/mL

View all Estrone ELISA Kits »

Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Estrone ELISA Kit is an enzyme immunoassay for the quantitative measurement of estrone.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.67%

Inter-Assay Precision (Precision between assays) CV% < 9.5%

Recovery for Human Estrone ELISA Kit (Colorimetric)

Recovery

99.47%

Scientific Data Images for Human Estrone ELISA Kit (Colorimetric)

ELISA: Human Estrone ELISA Kit (Colorimetric) [KA1908]

ELISA: Human Estrone ELISA Kit (Colorimetric) [KA1908]

ELISA: Estrone ELISA Kit [HRP] [KA1908] - Standard curve

Kit Contents for Human Estrone ELISA Kit (Colorimetric)

  • Assay Buffer: Ready to use. One vial containing a protein-based buffer with a non-mercury preservative.
  • Avidin-Horse Radish Peroxidase (HRP) Conjugate Concentrate (100x): Avidin-HRP conjugate in a protein-based buffer with a non-mercury preservative.
  • Estrone-Biotin Antibody Concentrate (100x): Estrone-Biotin Antibody in a protein-based buffer with a non-mercury preservative.
  • Rabbit Anti-Estrone Antibody Coated Microwell Plate: Break Apart Wells. Ready to use. One 96 well polyclonal antibody-coated microwell plate in a resealable pouch with desiccant.
  • Stopping Solution: Ready to use. One vial containing 1 M sulfuric acid.
  • TMB Substrate: Ready to use. One bottle containing tetramethylbenzidine and hydrogen peroxide in a non-DMF or DMSO containing buffer.
  • Wash Buffer Concentrate (10x): One bottle containing buffer with a non-ionic detergent and a non-mercury preservative.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Estrone

Estrone, estradiol, and estriol are all estrogens natural to the human body. They belong to the group of steroid hormones, and are mostly responsible for the growth of female characteristics in puberty and also regulation of the menstrual cycle. They are produced primarily in the ovaries but are also manufactured from androstenedione in fat cells, muscle cells, and skin. Oral estrogen is converted to estrone(less potent) by the gut and liver, reversing the normal premenopausal 2:1 ratio of 17b-oestradiol to estrone. Estrone Levels 1. Men 2.90 - 7.50 pg/0.1 ml 2. Women (days of menstrual cycle) 1-10 days 4.30 - 18.0 pg/0.1 ml 11-20 days 7.50 - 19.6 pg/0.1 ml 21-30 days 13.1 - 20.1 pg/0.1 ml

Alternate Names

Oestrone

Additional Estrone Products

Product Documents for Human Estrone ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Estrone ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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