Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

Name: Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP3-50070

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50070

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.5 pg/mL

Assay Range

62.5 - 4000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Mouse

Specificity

This kit recognizes Mouse ER alpha/NR3A1 in samples. No significant cross-reactivity or interference between Mouse ER alpha/NR3A1 and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

Recovery

80 - 120%

Linearity

Scientific Data Images for Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

ELISA: Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence) [NBP3-50070]

Standard Curve Reference.

Kit Contents for Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Certificate of Analysis
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate(100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Description
Reference Standard
Reference Standard & Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

Product Datasheets

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COA

Product Specific Notices for Mouse ER alpha/NR3A1 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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