Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75373
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
9.38 pg/mL
Assay Range
15.63 - 1000 pg/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Rat
Specificity
This kit recognizes Rat SDF-1(alpha) in samples. No significant cross-reactivity or interference between Rat SDF-1(alpha) and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 5.05%
Inter-Assay Precision (Precision between assays) CV% < 5.11%
Recovery for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
Recovery
85-107%
Linearity
Scientific Data Images for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
ELISA: Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric) [NBP2-75373] -
ELISA: Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric) [NBP2-75373] - Standard Curve ReferenceKit Contents for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CXCL12/SDF-1 alpha
CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
Additional CXCL12/SDF-1 alpha Products
Product Documents for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
Product Specific Notices for Rat CXCL12/SDF-1 alpha ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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