Mouse Complement C3d ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50048

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.08 ng/mL

Assay Range

2.34 - 150 ng/mL

View all Complement C3d ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse Complement Complement C3d in samples. No significant cross-reactivity or interference between Mouse C3d and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Mouse Complement C3d ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Mouse Complement C3d ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Complement C3d ELISA Kit (Colorimetric)

Mouse Complement C3d ELISA Kit (Colorimetric)

ELISA: Mouse Complement C3d ELISA Kit (Colorimetric) [NBP3-50048]

Standard Curve Reference.

Kit Contents for Mouse Complement C3d ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement C3d

C3d is a fragment of the major complement protein C3, C3d is formed as a result of the deactivation of C3b.Factor I in the presence of cofactors cleaves C3b to form iC3b and C3f, Factor I and/or other proteasesthen cleaves iC3b to form C3c and C3dg. C3dg is then further cleaved by tryptic enzymes to C3g andC3d. The C3 fragments are then eliminated from circulation by phagocytic cells in the liver. C3d has amolecular weight of 34kDa. Normally C3d is present in trace amounts in plasma, however, in diseasestates where rapid complement consumption is occurring levels of C3d can be elevated.

Alternate Names

AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, complement C3, complement component 3, complement component C3, CPAMD1acylation-stimulating protein cleavage product

Gene Symbol

C3

Additional Complement C3d Products

Product Documents for Mouse Complement C3d ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Complement C3d ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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