cAMP Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE002B
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Saliva (50 µL), Urine (10 µL)
Sensitivity
8.57 pmol/mL
Assay Range
3.75-240 pmol/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for cAMP Parameter Assay Kit
The Parameter cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, plasma, serum, saliva, and urine.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures cyclic AMP levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pmol/mL) | 22.6 | 68.7 | 116 | 21.7 | 70.4 | 119 |
| Standard Deviation | 1.37 | 2.55 | 2.54 | 2.2 | 6.0 | 7.3 |
| CV% | 6.0 | 3.7 | 2.2 | 10.3 | 8.6 | 6.1 |
Cell Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pmol/mL) | 18.5 | 56.9 | 96.1 | 17.0 | 51.8 | 89.2 |
| Standard Deviation | 0.89 | 1.74 | 2.73 | 1.3 | 3.5 | 6.9 |
| CV% | 4.8 | 3.1 | 2.8 | 7.4 | 6.8 | 7.7 |
Recovery for cAMP Parameter Assay Kit
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 86-119 |
| Cell Lysis Buffer (n=1) | 91 | 87-94 |
| EDTA Plasma (n=4) | 105 | 92-120 |
| Heparin Plasma (n=4) | 98 | 85-111 |
| Saliva (n=4) | 97 | 80-117 |
| Serum (n=4) | 103 | 97-114 |
| Urine (n=6) | 100 | 91-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for cAMP Parameter Assay Kit
Multi-species cAMP ELISA Cell Lysis Buffer 5 (1X) Standard Curve
Multi-species cAMP ELISA Calibrator Diluent RD5-55 Standard Curve
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP
cAMP (adenosine 3’5’-cyclic monophosphate) is a ubiquitous intracellular second messenger that mediates a diverse range of cellular processes in all organisms from bacteria to higher eukaryotes. It is converted from adenosine triphosphate (ATP) by adenylyl cyclases (ACs), and is inactivated by phosphodiesterases (PDEs) which catalyze its hydrolysis to 5’-AMP. In mammals, upon interaction with extracellular ligands, G protein coupled receptors (GPCRs) linked to Gαs activate the family of nine transmembrane ACs to increase intracellular cAMP. In contrast, GPCRs associated with Gαi/o inhibit the synthesis of cAMP by transmembrane ACs. cAMP binds and activates protein kinase A (PKA), the guanine nucleotide exchange factors Epac1/2, and cyclic nucleotide-gated ion channels. It can be released extracellularly where it is converted to adenosine by phosphodiesterases and nucleotidases.
Long Name
Cyclic AMP
Alternate Names
Cyclic AMP
Additional cAMP Products
Product Documents for cAMP Parameter Assay Kit
Product Specific Notices for cAMP Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.