Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-56256

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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.064 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

View all Aminopeptidase PILS/ARTS1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric)

Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric) ELISA: Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric) [NBP3-56256]

ELISA: Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric) [NBP3-56256]

Kit Contents for Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aminopeptidase PILS/ARTS1

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is a 120 kDa protein localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. Peptides that are presented by MHC class I on the surface of a cell must be 8-11 residues long, and ERAP1 specifically trims peptides of 9 amino acids or more. ERAP1 is also induced by interferon-gamma. The gene encoding human ERAP1 maps to chromosome 5q15. ERAP1 has previously been characterized as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). A-LAP is thought to inactivate several bioactive peptides, including angiotensin II and, subsequently, may be involved in the regulation of blood pressure. PILS-AP is described as playing a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and ARTS-1 is characterized as a TNFR1 binding protein that promotes TNFR1 shedding. Further research will be necessary to fully elucidate the functions of this protein.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Gene Symbol

ERAP1

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Aminopeptidase PILS/ARTS1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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