12S-HETE ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07908
Key Product Details
Sample Type & Volume Required Per Well
Plasma, culture supernates (100 ul)
Sensitivity
146 pg/ml
Assay Range
195 - 50000 pg/ml
Product Specifications
Assay Type
Indirect ELISA
Kit Type
ELISA Kit (Colorimetric)
Specificity
Cross-reactivities for a number of related compounds:
12(R)-HETE: 2.5%
15-HETE: 0.3%
5(S)-HETE: 0.2%
8,15-diHETE: 0.1%
5,15-diHETE: 0.1%
PGE2: 0.1%
PGF2alpha: 0.1%
PGD2: 0.1%
6-keto-PGF1alpha: 0.1%
Thromboxane B2: 0.1%
Arachidonic Acid: 0.1%
Leukotriene B4: 0.1%
Leukotriene C4: 0.1%
Leukotriene D4: 0.1%
Leukotriene E4: 0.1%
8-HETE: 0.1%
9-HETE: 0.1%
11-HETE: 0.1%
12(R)-HETE: 2.5%
15-HETE: 0.3%
5(S)-HETE: 0.2%
8,15-diHETE: 0.1%
5,15-diHETE: 0.1%
PGE2: 0.1%
PGF2alpha: 0.1%
PGD2: 0.1%
6-keto-PGF1alpha: 0.1%
Thromboxane B2: 0.1%
Arachidonic Acid: 0.1%
Leukotriene B4: 0.1%
Leukotriene C4: 0.1%
Leukotriene D4: 0.1%
Leukotriene E4: 0.1%
8-HETE: 0.1%
9-HETE: 0.1%
11-HETE: 0.1%
Description
Store all components at 4C, except standard and conjugate at -20C.
Precision
Intra-Assay Precision (Precision within an assay) CV%: <15%
Inter-Assay Precision (Precision between assays) CV%: <21%
Recovery for 12S-HETE ELISA Kit (Colorimetric)
Recovery
Culture Media: 94%
Sodium heparin plasma 104
Sodium heparin plasma 104
Scientific Data Images for 12S-HETE ELISA Kit (Colorimetric)
Non-species specific 12S-HETE ELISA Kit (Colorimetric) [NBP3-07908] - Standard curve
Kit Contents for 12S-HETE ELISA Kit (Colorimetric)
- 12(S)-HETE Antibody
- 12(S)-HETE Assay Layout Sheet
- 12(S)-HETE Conjugate
- 12(S)-HETE Standard
- Assay Buffer Concentrate
- Goat anti-Rabbit IgG Microtiter Plate
- Plate Sealer
- pNpp Substrate
- Stop Solution
- Wash Buffer Concentrate
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: 12S-HETE
Alternate Names
(5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid, 12(S)-HETE, 12(S)-Hydroxyeicosatetraenoic acid, 12-Hete, 12-Hydroxyeicosatetraenoic acid, 12S-HETE, 54397-83-0, CHEBI:34146
Additional 12S-HETE Products
Product Documents for 12S-HETE ELISA Kit (Colorimetric)
Product Specific Notices for 12S-HETE ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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