LinkAble™ PE-Cy7 Antibody Conjugation Kits New
R&D Systems, part of Bio-Techne | Catalog # LNKPC7
Key Product Details
Applications
Flow Cytometry
Assay Type
Antibody Conjugation Kit
Conjugate
PE/Cy7
Product Summary for LinkAble™ PE-Cy7 Antibody Conjugation Kits
LinkAble™ PE-Cy7 Antibody Conjugation Kits enable rapid, in house antibody labeling and conjugation to phycoerythrin-cyanine 7 (PE-Cy7) through primary amine groups (e.g. lysines). Designed for straightforward, in-house use, the PE-Cy7 conjugation kit supports reliable, reproducible conjugation with minimal hands-on time and no requirement for specialized expertise or additional purification steps. Conjugates are ready to use in approximately two hours, supporting efficient evaluation and comparison of antibodies in downstream assays.
The kits are optimized for single-use reactions and are also available in multipack formats to support higher-throughput or repeat experimental workflows.
Key Benefits
- Ease & Efficiency: Streamlined protocols ensure a simple, fast, and reliable conjugation process. The kit requires only 30 seconds of hands-on time and produces conjugates that are ready to use in 2 hours, without any loss of antibody associated with buffer exchange and purification.
- Reproducibility: Optimized reagents deliver consistent, high-quality results. The kit is available in 25 µg, 100 µg, and 1 mg antibody input sizes, which have been optimized to perform identically across all three sizes, allowing scaling of conjugation reactions.
- Streamlined & Optimized Usability: The ready-to-use format eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. An intuitive, user friendly design includes screw cap vials that can be easily centrifuged to recover the sample.
Starting Mass & Volume of Antibody
The kit produces high quality conjugates using IgG antibodies with a concentration of 250 µg/mL or greater. For optimal results, it is recommended to carry out the conjugation reaction with antibody concentration at 1 mg/mL.
| Reaction Size | Minimum (μg) | Optimal (μg) | Maximum (μg) |
|---|---|---|---|
| 25 μg | 10 | 25 | 100 |
| 100 μg | 25 | 100 | 400 |
| 1000 μg | 250 | 1000 | 4000 |
Technical Hints & Limitations
- Although it is recommended to use a purified antibody in a suitable buffer (e.g. PBS) and free from additives, the LinkAble™ PE-Cy7 Antibody Conjugation Kit produces high quality conjugates in the presence of some buffers/additives.
Note: The kit is not compatible with certain components, as described in the table below.
| Components | Compatible |
|---|---|
| Amine-free buffer (MES, PBS, HEPES, bicarb, borate) | Yes |
| Tris | ≤ 100 mM |
| Sugars (trehalose / sucrose) | Yes |
| EDTA | Yes |
| Proclin | Yes |
| Sodium azide | No |
| BSA | ≤ 0.1% |
| Glycerol | ≤ 10% |
| Amine-containing reagents (glycine, arginine) | No |
- If multiple, non-optimal parameters are used in the conjugation reaction (concentration, additives, etc.) poor quality conjugates may result.
- Centrifuge all vials before resuspending to ensure all contents are at the bottom of the vials.
- In each step, gently pipette the sample up and down to mix.
- Purification of the conjugated antibody is not necessary, though a user may choose to do so.
Scientific Data Images for LinkAble™ PE-Cy7 Antibody Conjugation Kits
Enhanced Performance of CD3-PE-Cy7 Conjugates Prepared Using LinkAble™ PE-Cy7 Antibody Conjugation Kit Versus Other Supplier Kit
Mouse anti-CD3 (MAB100) was conjugated using LinkAble™ PE-Cy7 Antibody Conjugation Kit and a PE-Cy7 conjugation kit from another supplier. Human peripheral blood lymphocytes were stained following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
High Reproducibility of CD3-PE-Cy7 Conjugates Made Using LinkAble™ PE-Cy7 Antibody Conjugation Kits
Three Mouse anti-CD3 (MAB100) conjugates were prepared using LinkAble™ PE-Cy7 Antibody Conjugation Kits and then used to stain human peripheral blood lymphocytes following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
Consistent Batch-to-Batch Performance of CD3-PE-Cy7 Conjugates Made Using LinkAble™ PE-Cy7 Antibody Conjugation Kits
Mouse anti-CD3 (MAB100) PE-Cy7 conjugates were prepared using three independent batches of LinkAble™ PE-Cy7 Antibody Conjugation Kits and then used to stain human peripheral blood lymphocytes following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for LinkAble™ PE-Cy7 Antibody Conjugation Kits
Product Specific Notices for LinkAble™ PE-Cy7 Antibody Conjugation Kits
LinkAbleTM is a trademark of Bio-Techne.
Wear appropriate protective gloves, clothing, and eye/face protection. Wash hands thoroughly after handling. Consult the Safety Data Sheet (SDS) on our website prior to use. Review SDS information for all kit components, including the borate buffer.
For research use only
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