Can I label a peptide/protein/antibody other than IgG?

Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is half the size of an antibody (about 80kD), add half as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10ug kits enable you to do this using small amounts of material and therefore at a low cost.

Can I use your lightning-link kits to label other proteins rather than antibodies?

Yes, the kits work by using free amines, so as long as you have that the kit should work.

Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

Do I need a wash or desalt step?

One of the advantages of the Lightning-Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation. Any remaining free label would have its reactive 'Lightning-Link®' groups blocked by the Quencher provided in the kit, and would then be washed away during the relevant wash step of your application.

Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Do my antibody and buffer fit the requirements?

Your antibody should be purified (affinity purification is preferred), as other molecules with free amine groups will interfere with the reaction, resulting in a poor quality conjugate. A suitable method of purification should be used (such as affinity or Protein A/G). Please note that 0.2/0.22 um filtration is a method of sterilization, not purification. Your biomolecule should also be in a suitable, 10-50mM amine free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5 and not in any of the following: ascites fluid, serum or tissue culture supernatant. It should not contain any additives such as Azide, BSA, Tris or Glycine at a concentration of >0.1%.The amount of antibody (IgG) you should add to the Lightning-Link® vial usually corresponds to the kit size your purchased (for example: a 3x100ug kit enable you to label 3 lots of 100ug antibody) and the volume added should also match (eg: 100ul), meaning the ideal concentration is of 1mg/ml. For other biomolecule, the amount added should be adjusted by changing your concentration (see above).NOTE: The amount and volume of antibody recommended above are for all Lightning-Link• kits offered by Innova Biosciences, with the exception of RPE and PE tandem dyes. For more details on the recommendations specific to these dyes, please consult the protocols available on each product page.

How many labels will bind to my biomolecule?

It is difficult to give a precise answer to this as the result will vary from antibody to antibody (or other protein). Traditional labelling techniques often have F/P ratios in the range of 4-7:1, and our comparative data for staining with antibodies labelled with Lightning Link shows similar staining intensity. The ratio of dye to antibody is only ever an average for the population of labelled molecules, as individual molecules may have different amount of dye incorporated into them.

How stable will my new conjugate be?

The Lightning-Link® chemistry joins the label to the antibody via a uni directional stable, covalent bond. The stability of your conjugate will therefore be dependent on your antibody and label of choice. In our in-house studies, conjugates made with our Lightning-Link® kits were fully active after more than 18 months' storage, undiluted, at 4 degrees C. Conjugates stored in 50% glycerol at -20 degrees C were found to be even more stable (several years). Please see below for advice on storage conditions. Please note that tandem dye stability is lower than the other dyes'. Tandem conjugates are fully stable for about 3 months at 4 degrees C.

Is trehalose accepatable in PBS for label with Lightning Link R-PE

The trehalose should not affect any protein modification. Trehalose is a common component as lyophilised excipient and we do not expect that it will interfere with the conjugation.

What are the best storage conditions for my new conjugate?

TA new conjugate can be stored for 12-18 months at 4 degrees C as long as the antibody will tolerate storage at 4 degrees C. As the bond between the antibody and dye is covalent and very stable, it should not degrade, therefore at 4 degrees C no additional preservatives are needed. The antibody is usually the least stable component of the conjugate so please check the antibody datasheet for storage recommendations. However, all conjugates can be stored in 50% glycerol at -20 degrees C which allows them to remain stable for 2 years. Please bear in mind that APC and RPE conjugates should never be stored at -20 degrees C on their own without glycerol. The dilution factor also has an effect. Storing the conjugate undiluted is recommended if possible; however, for HRP conjugates, if you wish to store at working concentrations (e.g. 1/10,000), this can be done using our LifeXtend HRP Conjugate Stabilizer/Diluent. Please note that the preservative sodium azide will inhibit HRP. We suggest that the optimal storage conditions for any conjugate are determined experimentally, using small aliquots of the conjugate.

What can I do if my antibody formulation does not fit the requirements?

If your antibody buffer is not compatible with our kits, we have developed an AbSelect™ purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with the Lightning-Link® kits.The appropriate kit to use depends on your particular sample (species, buffer, contaminants, volume,...). We have designed a handy flow chart on the AbSelect™ webpage to help you select a kit, but feel free to contact us if you require further guidance. Please consult the kit protocols to see the antibody amount/volume suitable for each kit.If your antibody is already purified but its concentration is too low, you can concentrate it by using our Antibody Concentration and Clean Up Kit. This kit can also be used to remove low molecular weight contaminants such as azide, tris or glycine by carrying out a buffer exchange into the buffer supplied in the kit, which is fully compatible with Lightning-Link®.If your antibody contains BSA, you can now use our BSA removal kit to purify your antibody in a few simple steps. Please note this kit will also enable you to concentrate your antibody.NB: All the AbSelect kits will ONLY work with antibodies. They will not purify other molecules. The only exception is the concentration and clean up kit (861-0010), which will work with other molecules greater than 10kD.

What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

What is the difference between Lightning-Link® and Lightning-Link® Rapid?

The benefit of the Rapid kits is that the conjugates can be generated faster because the conjugation and quenching incubation times are shorter. The antibody/protein/peptide considerations are exactly the same, as is the high quality of the final conjugate. DyLight® dyes are only available in the Rapid format.

Lightning-Link (R) R-PE Antibody Labeling Kit

Name: Lightning-Link (R) R-PE Antibody Labeling Kit
Brand: Novus Biologicals
SKU: 703-0005

Novus Biologicals, part of Bio-Techne | Catalog # 703-0005

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
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Specific Notices

Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

R-Phycoerythrin

Product Summary for Lightning-Link (R) R-PE Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our R-PE antibody labeling kit enables the direct conjugation of R-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours.

Features	Benefits
Quick and easy to use	Save time, no special knowledge required
No separation steps	100% recovery - no antibody/protein loss
Can be used in a wide range of applications	Flexible
Freeze dried	Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available	Experimental flexibility
Reliable: nearly 300 references	Successfully used in many fields of research

R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, present in red algae and cryptophytes. It has three maximal absorbance values of 498, 544 and 566nm (the optimal will depend on the application), and it has a strong emission peak at 580nm. RPE is closely related to B-Phycoerythrin (B-PE) and these are the most intense fluorescent phycobiliproteins providing an orange fluorescence.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit can be used to label up to 60 ug of antibody, and is supplied in one vial.

Scientific Data Images for Lightning-Link (R) R-PE Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Flow Cytometry: Lightning-Link R-PE Antibody Labeling Kit [703-0005] - Direct labeling with Lightning Link CD45RC-PE

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Flow Cytometry: Lightning-Link R-PE Antibody Labeling Kit [703-0005] - Indirect labeling with CD45RC supernatant

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Flow Cytometry: Lightning-Link R-PE Antibody Labeling Kit [703-0005] - Indirect labeling with purified CD45RC antibody

Kit Contents for Lightning-Link (R) R-PE Antibody Labeling Kit

1 or 3 or 5 glass vial(s) of Lightning-Link mix
1 vial of LL-Modifier reagent
1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) R-PE

Easy R-PE labeling of your antibody with 30 seconds hands-on time. R-PE (R-Phycoerythrin) is a large 240kDa red phycobiliprotein isolated from red algae. R-PE has a strong absorption peak at 565nm and a secondary absorption peak at 492nm. The R-PE protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting. The Lightning-Link labeling system represents a quantum leap forward in antibody labeling technology. It allows the researcher to make labeled antibodies with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality labeled antibodies and proteins. As the procedure is not interrupted by desalting steps, trial labels can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Additional Lightning-Link (R) R-PE Products

Product Documents for Lightning-Link (R) R-PE Antibody Labeling Kit

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Lightning-Link (R) R-PE Antibody Labeling Kit

COA

SDS

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Product Specific Notices for Lightning-Link (R) R-PE Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Lightning-Link (R) R-PE Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 703-0005

Key Product Details

Applications

Kit Type

Conjugate

Product Summary for Lightning-Link (R) R-PE Antibody Labeling Kit

Product Specifications

Application Notes

Scientific Data Images for Lightning-Link (R) R-PE Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0005]

Kit Contents for Lightning-Link (R) R-PE Antibody Labeling Kit

Formulation, Preparation, and Storage

Concentration

Shipping

Storage

Background: Lightning-Link (R) R-PE

Additional Lightning-Link (R) R-PE Products

Product Documents for Lightning-Link (R) R-PE Antibody Labeling Kit

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Lightning-Link (R) R-PE Antibody Labeling Kit

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