LATEX 400nm Blue Mini Antibody Conjugation Kit
Novus Biologicals, part of Bio-Techne | Catalog # 1000-0040
Key Product Details
Kit Type
Antibody Conjugation Kit
Product Summary for LATEX 400nm Blue Mini Antibody Conjugation Kit
The LATEX Conjugation Kit allows antibodies or proteins to be conjugated to our high quality 400nm blue latex beads quickly and easily. The latex beads are designed for ease of use and have much improved handling compared with traditional latex. This has been achieved by specially treating the particles. The conjugation is covalent, and requires less antibody than traditional passive and covalent latex conjugates.
LATEX Conjugation Procedure: 30 seconds to set up the conjugation 3 minutes hands-on time for the conjugation procedure Conjugate is ready to use within 35 minutes. Simply pipette the biomolecule into a vial containing the latex nanoparticles, then centrifuge to buffer exchange.
LATEX Conjugation Procedure: 30 seconds to set up the conjugation 3 minutes hands-on time for the conjugation procedure Conjugate is ready to use within 35 minutes. Simply pipette the biomolecule into a vial containing the latex nanoparticles, then centrifuge to buffer exchange.
Product Specifications
Application Notes
We would suggest that the antibody to be conjugated should be in 10 - 50 mM MES, HEPES or MOPS at pH 6 - 7 (with no other components e.g. salt or azide) before conjugation.
Scientific Data Images for LATEX 400nm Blue Mini Antibody Conjugation Kit
LATEX 400nm Blue Mini Antibody Labeling Kit [1000-0040] - Lateral Flow Assay of specially-treated latex beads conjugated to a monoclonal anti-CRP antibody (mAb1) titrated against CRP. 2 ng/ml CRP can easily be detected.
LATEX 400nm Blue Mini Antibody Labeling Kit [1000-0040] - Lateral Flow Assay of specially-treated latex conjugated to the monoclonal anti-CRP antibody (mAb 2) titrated against CRP in buffer or CRP spiked CRP-depleted serum (100 % serum). The latex behaves similarly in serum and buffer, and no aggregation or non-specific binding is seen.
Kit Contents for LATEX 400nm Blue Mini Antibody Conjugation Kit
- 1 vial of 10x Quencher buffer
- 1 vial of Reaction buffer A
- 1 vial of Reaction buffer B
- 1 vial of Resuspension buffer
- 4 or 10 Mini vials of 400nm Blue/Red/ or Black LATEX (dependent on kit)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C.
Background: LATEX 400nm Blue Mini
Additional LATEX 400nm Blue Mini Products
Product Documents for LATEX 400nm Blue Mini Antibody Conjugation Kit
Product Specific Notices for LATEX 400nm Blue Mini Antibody Conjugation Kit
LATEX® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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