MagCellect Mouse Naive CD4+ T Cell Isolation Kit

For the isolation of mouse naïve CD4⁺ T cells.

Why Isolate Naïve CD4⁺ T cells?

Mouse CD4⁺ T cells develop in the thymus and are released as naïve T cells that also express CD62L and low levels of CD44. These cells differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental cues and the activation of specific transcription factors. The isolation of CD4⁺ T cells is an important step in the preparation of multiple subsets including Th1, Th2, Th17, Th9, Th22, and regulatory T cells. CD4⁺ T cell subsets secrete characteristic combinations of cytokines which enables them to exert diverse functions including the recruitment and activation of additional immune cells, the dampening of ongoing immune responses, and the maintenance of immunologic memory.

Reagents Provided

The MagCellect Mouse Naïve CD4⁺ T Cell Isolation Kit contains the following reagents that allow for isolation of highly pure mouse naïve CD4⁺ T cells.

• MagCellect Mouse Naïve CD4⁺ T Cell Biotinylated Antibody Cocktail
• MagCellect Streptavidin Ferrofluid
• MagCellect Plus Buffer (10X)

This kit contains sufficient reagents to process 1 x 10⁹ total cells.

Stability and Storage

Store all reagents at 2 °C to 8 °C. DO NOT FREEZE.