DNA transposon component of the TcBuster non-viral gene delivery system.
Key Product Details
Features:
TcBuster Transposon Insert On eGFP is a DNA transposon (plasmid) containing an eGFP sequence flanked by TcBuster-compatible ITR sequences that is only expressed after genomic integration. As a component of the TcBuster non-viral gene delivery system, this product requires TcBuster-M Transposase mRNA to function. This two component system can be introduced, by electroporation, into a wide variety of cell types to enable stable gene transfer.
Key Benefits:
Used as an eGFP-expressing control for the TcBuster system in both cell therapy and bioproduction applications
Based on a genetic switch, expression of eGFP only occurs after transposition and is a direct readout of cargo (eGFP) integration
What is the TcBuster Non-Viral Gene Delivery System?
Gene delivery is a crucial step for a wide variety of genome engineering workflows, which have traditionally utilized viral vectors to achieve expression of exogenous DNA sequences. However, the generation of a virus is time consuming, inconsistent, and has limited cargo capacity. The TcBuster non-viral gene delivery system is a solution for stable integration of DNA cargo and addresses many of the limitations of viral vectors. It consists of the TcBuster-M Transposase mRNA and a compatible DNA TcBuster transposon, such as TcBuster Transposon Insert On eGFP. This product requires TcBuster Transposase mRNA to be functional.
Why use TcBuster Transposon Insert On eGFP?
This transposon encodes DNA cargo consisting of an eGFP sequence regulated by the EF1α promoter. It also incorporates a genetic switch, called integration-coupled On, that effectively eliminates expression of the untransposed plasmid (1). Gene expression can only occur after transposition and is a direct readout of cargo integration. When used in combination with TcBuster-M transposase mRNA, the DNA cargo will be inserted randomly into the host cell genome. This transposon serves as an ideal DNA cargo for initial evaluations of the TcBuster system and/or a control for cell therapy and bioproduction applications using the TcBuster system.
(1) Kumamoto, T. et al. .2020. Neuroresource
Limitations
For laboratory research use only. Not for use in diagnostic procedures.
For certain applications, results may vary due to differences in donor-derived cell populations.
Scientific Data Examples for TcBuster™ Transposon Insert On eGFP
TcBuster Transposon Insert On eGFP Plasmid Map.
HEK293E cells edited with the TcBuster system express eGFP.
HEK293E cells were gene edited with TcBuster-M transposase (Catalog # TCB-001.1-100) and Insert-On-eGFP transposon (Catalog # TCBP002-100). On day 2 after gene editing, cells were analyzed by flow cytometry for expression of eGFP. Representative flow cytometry plots of HEK293E cells edited with or without TcBuster-M transposase.
CHO-K1 cells edited with the TcBuster system express eGFP.
CHO-K1 cells were gene edited with TcBuster-M transposase (Catalog # TCB-001.1-100) and Insert-On-eGFP transposon (Catalog # TCBP002-100). On day 2 after gene editing, cells were analyzed by flow cytometry for expression of eGFP. Representative flow cytometry plots of CHO-K1 cells edited with or without TcBuster-M transposase.
Preparation & Storage
Shipping Conditions
This product ships at ≤ -20 °C. Upon receipt, store it immediately at the recommended temperature.
Storage
Store at ≤ -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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