Endothelial Cell Growth Media without VEGF

Kit Summary

Media for the low-serum, VEGF-free expansion of primary endothelial cells.

Why Use VEGF-free, low-serum Media to Grow Endothelial Cells?

Primary endothelial cells are commonly used for the study of developmental and disease mechanisms involving the blood vessels, such as angiogenesis. Endothelial cells are phenotypically characterized as CD31⁺, KDR⁺, and vWF⁺ cells.

Inclusion of Vascular Endothelial Growth Factor (VEGF) in endothelial cell media promotes cell proliferation. Under certain experimental conditions, a VEGF-free endothelial media may be beneficial. R&D Systems^® Endothelial Cell Growth Media without VEGF is formulated to support the expansion of human primary endothelial cells under low-serum, VEGF-free conditions.

This media has been tested for its ability to support CD31⁺, KDR⁺, and vWF⁺ human umbilical vein endothelial cells growth in vitro.

Kit Contents

This kit contains the following reagents to maintain and expand human and mouse endothelial cells in culture:

Containing optimized concentrations of:

• Endothelial Cell Growth Base Media (250 mL)
• Endothelial Cell Growth Supplement without VEGF (50X)
  • Human FGF basic
  • Human LR3 IGF-1
  • Human EGF
  • Heparin
  • Hydrocortisone
  • L-Ascorbic Acid
  • Fetal Bovine Serum

Note: The components for this kit require different storage/shipping temperatures and will arrive in separate packaging.

Stability and Storage

Store in the dark at < -20 °C in a manual defrost freezer. Do not use past the expiration date.

Precaution

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.
• Results may vary due to variations among cells derived from different donors.