CellXVivo Human NK Cell Expansion Kit
R&D Systems, part of Bio-Techne | Catalog # CDK015
For the expansion of human CD3-CD56+ NK Cells from peripheral blood mononuclear cells (PBMCs).
Key Product Details
Species
Human
Product Summary for CellXVivo Human NK Cell Expansion Kit
Kit Summary
For the expansion of human CD3-CD56+ NK Cells from peripheral blood mononuclear cells (PBMCs).
Key Benefits
- Contains high quality bioactive cytokines and antibodies
- Provides optimized reagents needed induce expansion of CD3-CD56+ NK cells
- Can use PBMCs as the starting cell population
- Kit-expanded NK cells are cytotoxic
- Does not require specialized instrumentation
Why Expand NK Cells Ex Vivo?
Natural Killer (NK) cells were identified as a distinct sub-population of lymphocytes that have the capacity to spontaneously lyse tumor cells. Human NK cells are phenotypically characterized by the expression of CD56 and the absence of CD3. NK cells produce immunoregulatory cytokines, including interferon-γ (IFN-γ), tumor necrosis factor-beta (TNF-β), tumor necrosis factor-α (TNF-α), granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin (IL)-10, and IL-13. Activation of NK cells can be triggered via NKp46 and NKp30 receptors or after stimulation with combinations of IL-2, IL-12, and IL-15. NK cells play an important role in both the adaptive and innate immune responses that govern infection, autoimmunity, and tumor immunosurveillance. Preclinical evidence and early clinical success has established NK cell immunotherapy as a promising therapeutic strategy in cancer. The ability to expand NK cell populations ex vivo is valuable to researchers who require NK cells for downstream applications including studies for cell therapy and cancer immunotherapy.
Reagents Provided
This kit contains the following reagents for the ex vivo expansion of human NK cells.
- Human Killer Cell Base Media 2
- NK Cell Expander 1
- NK Cell Expander 2
- NK Cell Expander 3
- NK Cell Expander 4
- NK Cell Expander 5
- Reconstitution Buffer 1
- Reconstitution Buffer 2
- 20X Wash Buffer
Note: The components for this kit require different storage/shipping temperatures and will arrive in separate packaging.
Stability and Storage
Store the unopened kit at < -20 °C. Do not use past the kit expiration date.
*Provided this is within the expiration date of the kit.
Precaution
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Limitations
- FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the label.
- Results may vary due to variations among cells derived from different donors.
Scientific Data Images for CellXVivo Human NK Cell Expansion Kit
Human NK Cell Expansion Using the CellXVivo™ Kit is Equivalent to Standard Feeder Cell Expansion.
Human NK cells were enriched from peripheral blood mononuclear cells and expanded (3 x 106starting population) using the CellXVivo™NK Cell Expansion Kit or the standard method using artificial antigen-presenting K562 clone 9.mbIL-21 cells. NK cell expansion was quantified at days 1, 7, 10, and 14. The rate of NK cell expansion observed using the CellXVivo™Kit was similar to that observed using K562 cells.Data courtesy of the laboratory of Dr. Bruce Walcheck at the University of Minnesota.
Kit-expanded NK Cells are Cytotoxic to Tumor Cells.
NK Cells expanded using the CellXVivo™NK Cell Expansion Kit were assessed for toxicity against the NK cell-sensitive K562 tumor cell line. K562 cell were loaded with the live-cell dye, Calcein-AM (Tocris, Catalog # 5119) prior to being mixed with NK cells for 4 hours at the indicated effector-to-target cell (E:T) ratios (A).
Flow Cytometry Analysis of Expanded Human NK Cells.
Human PBMCs were culturedin vitrofor 14 days to expand Natural Killer cells using reag ents included in the CellXVivo™Human NK Cell Expansion Kit. Compared to unexpanded PBMCs (A), PBMCs treated with the kit (B) show an increased number of CD3-CD56+NK cells. Flow cytometric analysis was performed on day 14 of the expansion and cells were stained with Human NCAM-1/CD56 PE-conjugated Antibody, and Human CD3ε PerCP-conjugated Antibody. Quadrants were set based on isotope-stained samples.
Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Natural Killer (NK) Cell Kits
Natural Killer (NK) cells are a distinct sub-population of lymphocytes that have the capacity to lyse tumor cells. Human NK cells are characterized by the expression of CD56, the absence of CD3, and the secretion of cytokines, including IFN-gamma, TNF-beta, TNF-alpha, GM-CSF, IL-10, and IL-13. Cytokine-induced killer (CIK) cells exhibit phenotypic and functional similarities to both T cells and NK cells. While CIK cells express CD3 and expand readily in culture like T cells, they do not require functional priming for in vivo activity, a feature shared with NK cells. CD3+CD56+ CIK cells have potent cytotoxic functions and express a number of cytokines, including IL-2, IFN-gamma, TNF-alpha, and GM-CSF. The ability to expand CIK or NK cell populations ex vivo is valuable to researchers who require these cells for downstream applications including studies for cell therapy and cancer immunotherapy.
Long Name
Natural Killer Cell Expansion
Additional Natural Killer (NK) Cell Kits Products
Product Documents for CellXVivo Human NK Cell Expansion Kit
Product Specific Notices for CellXVivo Human NK Cell Expansion Kit
For research use only
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