Proteome Profiler Human sReceptor Array, Non-hematopoietic
R&D Systems, part of Bio-Techne | Catalog # ARY012
Key Product Details
Summary for Proteome Profiler Human sReceptor Array, Non-hematopoietic
Kit Summary
To simultaneously detect the relative levels of 119 different soluble receptors expressed and released by non-hematopoietic cells in a single sample. No specialized equipment is necessary.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or cell lysate samples are diluted and incubated with each array part of the Non-Hematopoietic Panel. This panel is comprised of the Non-Hematopoietic Array and the Common Analytes Array. The membranes are washed to remove unbound material followed by incubation with their specific cocktail of biotinylated detection antibodies. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of receptor bound. Chemiluminescence is detected in the same manner as a Western blot.
- 2 Non-Hematopoeitic Array Membranes
- 2 Common Analyte Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these soluble receptors in a single sample. | |||||
| Non-Hematopoeitic Array | Common Analytes Array | ||||
| ADAM15 | Integrin alpha 6/CD49f | ACE | HB-EGF | ||
| betaIG-H3 | Integrin alpha 9 | ADAM8 | ICAM-2/CD102 | ||
| BMPR-IB/ALK-6 | Integrin alpha V/CD51 | ADAM9 | IL-1 RII | ||
| Cadherin-4/R-Cadherin | Jagged 1/CD339 | ADAM10 | IL-15 R alpha | ||
| Cadherin-11 | JAM-B/VE-JAM/JAM2/CD322 | ALCAM/CD166 | Integrin beta 1/CD29 | ||
| Cadherin-13 | JAM-C/JAM3 | Amphiregulin | Integrin beta 2/CD18 | ||
| E-Cadherin | LRP-6 | APP (pan) | Integrin beta 3/CD61 | ||
| N-Cadherin | MCAM/CD146 | BACE-1 | Integrin beta 4/CD104 | ||
| P-Cadherin | MEPE | BCAM/CD239 | Integrin beta 5 | ||
| VE-Cadherin | MUCDHL | C1q R1/CD93 | Integrin beta 6 | ||
| Cathepsin D | Nectin-2/CD112 | CD9 | JAM-A/CD321 | ||
| CD40 | Nectin-4 | CD23/Fc epsilon RII | Lipocalin-2/NGAL | ||
| CEACAM-5/CD66e | Neurotrimin | CD31/PECAM-1 | LOX-1/SR-E1/CLEC8A | ||
| CHL-1/L1CAM-2 | Notch-1 | CD36/SR-B3 | MD-1/LY86 | ||
| Clusterin | NrCAM | CD40 Ligand | MMP-2 (total) | ||
| Coagulation Factor II/Thrombin | Periostin/OSF-2 | CD44H | NCAM-1/CD56 | ||
| COMP/Thrombospondin-5 | Podocalyxin | CD58/LFA-3 | NCAM-L1/CD171 | ||
| CRELD2 | E-Selectin | CD90/Thy1 | Osteopontin | ||
| Desmoglein 2 | Semaphorin 3A | CD99 | PAR1 | ||
| ECM-1 | SREC-I/SR-F1 | CD155/PVR | Pref-1/DLK-1/FA1 | ||
| EGF R/ErbB1/HER1 | SREC-II | CEACAM-1/CD66a | RECK | ||
| Endoglycan | Stanniocalcin 1 | CX3CL1/Fractalkine | Stabilin-1 | ||
| EpCAM/TROP-1 | Syndecan-1/CD138 | CXCL8/IL-8 | TACE/ADAM17 | ||
| ErbB2/HER2 | Syndecan-4 | EMMPRIN/CD147 | Thrombospondin-1 | ||
| ErbB3/HER3 | Thrombospondin-2 | Endoglin/CD105 | TIMP-1 | ||
| ErbB4/HER4 | TIMP-4 | Epiregulin | TIMP-2 | ||
| ESAM | TROP-2 | Galectin-1 | TIMP-3 | ||
| Galectin-2 | VAP-1/AOC3 | Galectin-3 | TNF RII | ||
| HPRG | VCAM-1 | Galectin-3BP/MAC-2BP | |||
| Integrin alpha 3/CD49c | VEGF R1/Flt-1 | ||||
| Integrin alpha 5/CD49e | VEGF R2/KDR/Flk-1 | ||||
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