ZNF174 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86821
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ZNF174 Antibody - BSA Free (NBP1-86821) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ZNF174 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZNF174 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody [NBP1-86821]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86821-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody [NBP1-86821]")
Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody [NBP1-86821]
Immunocytochemistry/Immunofluorescence: ZNF174 Antibody [NBP1-86821] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & actin filaments.![Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-Immunohistochemistry-Paraffin-NBP1-86821-img0006.jpg "Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821]")
Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821]
Immunohistochemistry-Paraffin: ZNF174 Antibody [NBP1-86821] - Staining of human gallbladder shows moderate nuclear positivity in glandular cells.![ZNF174 Antibody - BSA Free Western Blot: ZNF174 Antibody - BSA Free [NBP1-86821]](https://resources.bio-techne.com/images/products/nbp1-86821_rabbit-polyclonal-znf174-antibody-304202513135591.jpg "Western Blot: ZNF174 Antibody - BSA Free [NBP1-86821]")
Western Blot: ZNF174 Antibody - BSA Free [NBP1-86821]
Analysis in control (vector only transfected HEK293T lysate) and ZNF174 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Applications for ZNF174 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF174
Alternate Names
AW-1, zinc finger protein 174, ZSCAN8Zinc finger and SCAN domain-containing protein 8
Gene Symbol
ZNF174
Additional ZNF174 Products
Product Documents for ZNF174 Antibody - BSA Free
Product Specific Notices for ZNF174 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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