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ZEB2 Antibody (CL13467) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24559

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NBP3-24559-100ul
NBP3-24559-25ul
Conjugate
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13467

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O60315, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ZEB2 Antibody (CL13467) - BSA Free

Immunohistochemistry-Paraffin: ZEB2 Antibody (CL13467) [NBP3-24559]

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: ZEB2 Antibody (CL13467) [NBP3-24559]

Staining of human tonsil shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: ZEB2 Antibody (CL13467) [NBP3-24559]

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in tumor cells.

Applications for ZEB2 Antibody (CL13467) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1ug/ml
Application Notes
IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB2/SIP1

The ZEB2 gene (also known as SIP1 and SMADIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Alternate Names

HSPC082, SIP1, SMADIP1, ZFHX1B

Gene Symbol

ZEB2

Additional ZEB2/SIP1 Products

Product Documents for ZEB2 Antibody (CL13467) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZEB2 Antibody (CL13467) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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