ZEB1 Antibody (2A8A6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81015
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0008.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
![Western Blot: ZEB1 Antibody (2A8A6)Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Western-Blot-NBP2-81015-img0001.jpg "Western Blot: ZEB1 Antibody (2A8A6)Azide and BSA Free [NBP2-81015]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Cited:
CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2A8A6
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Partial recombinant human ZEB1 (between residues 800-1124) expressed in E. coli [Uniprot P37275]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ZEB1 Antibody (2A8A6) - Azide and BSA Free
Western Blot: ZEB1 Antibody (2A8A6)Azide and BSA Free [NBP2-81015]
Western Blot: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-81015-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81015-img0004.jpg "Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]
Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining. Image from the standard format of this antibody.![Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81015-img0003.jpg "Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0005.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0006.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0007.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015]")
Applications for ZEB1 Antibody (2A8A6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1: 500 - 1:2000. Use reported by customer review and in scientific literature (PMID 29383119)
Application Notes
This antibody has had weak results in Western blot.
Reviewed Applications
Read 1 review rated 4 using NBP2-81015 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (2A8A6) - Azide and BSA Free
Product Specific Notices for ZEB1 Antibody (2A8A6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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