XPE/DDB2 Antibody (24E5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26631
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 24E5 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human XPE/DDB2 [UniProt Q92466]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit XPE/DDB2 Antibody (24E5) - BSA Free (NBP3-26631) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for XPE/DDB2 Antibody (24E5) - BSA Free
Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] -
Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Image of XPE/DDB2 Antibody (24E5) diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB.
Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] -
Immunohistochemistry: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Image of XPE/DDB2 Antibody (24E5) diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.46% DAB.
Western Blot: XPE/DDB2 Antibody (24E5) [NBP3-26631] -
Western Blot: XPE/DDB2 Antibody (24E5) [NBP3-26631] - Positive Western Blot detected in: PC-3 whole cell lysate.All lanes: XPE/DDB2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Applications for XPE/DDB2 Antibody (24E5) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: XPE/DDB2
Long Name
Xeroderma Pigmentosum E
Alternate Names
DDB2, XP5
Gene Symbol
DDB2
Additional XPE/DDB2 Products
Product Documents for XPE/DDB2 Antibody (24E5) - BSA Free
Product Specific Notices for XPE/DDB2 Antibody (24E5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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