WT1 Antibody (CL13165) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18564
![Western Blot: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Western-Blot-NBP3-18564-img0010.jpg "Western Blot: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0009.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL13165
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P19544, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for WT1 Antibody (CL13165) - BSA Free
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.![Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunocytochemistry-Immunofluorescence-NBP3-18564-img0001.jpg "Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]")
Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]
Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0003.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0005.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0007.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]](https://resources.bio-techne.com/images/products/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0008.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]")
Applications for WT1 Antibody (CL13165) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WT1
Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.
Long Name
Wilms Tumor 1
Alternate Names
GUD, WAGR, WIT-2, WT33
Gene Symbol
WT1
Additional WT1 Products
Product Documents for WT1 Antibody (CL13165) - BSA Free
Product Specific Notices for WT1 Antibody (CL13165) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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