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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2A Clone # 325004

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-5b
Ser55-Ser95 and Lys246-Glu315 connected by a Gly-Ser linker
Accession # NP_033551

Specificity

Detects mouse Wnt-5b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-5a, -8a, -8b, -9b, -10a, -10b, or recombinant human Wnt-7b is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Applications for Wnt-5b Antibody (325004) [CoraFluor™ 1]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Wnt-5b

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteinshave been implicated in oncogenesis and in several developmental processes, including regulation of cell fate andpatterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94%and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicingof this gene generates 2 transcript variants. (provided by RefSeq)

Long Name

Wingless-type MMTV Integration Site Family, Member 5b

Alternate Names

Wnt5b

Gene Symbol

WNT5B

Additional Wnt-5b Products

Product Documents for Wnt-5b Antibody (325004) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-5b Antibody (325004) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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