VPS35 Antibody (7E4) - BSA Free

Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.

Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.