VPS33A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20872
![Knockdown Validated: VPS33A Antibody [NBP2-20872]](https://resources.bio-techne.com/images/products/VPS33A-Antibody-Knockdown-Validated-NBP2-20872-img0007.jpg "Western Blot: VPS33A Antibody [NBP2-20872]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (97%), Chimpanzee (100%), Porcine (97%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VPS33A. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (83%), Xenopus laevis (86%).
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Lysosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit VPS33A Antibody - BSA Free (NBP2-20872) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-VPS33A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VPS33A Antibody - BSA Free
![Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872]](https://resources.bio-techne.com/images/products/VPS33A-Antibody-Immunohistochemistry-Paraffin-NBP2-20872-img0002.jpg "Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872]")
Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872]
Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872] - Immunohistochemical analysis of paraffin-embedded colon ca, using antibody at 1:500 dilution.
Western Blot: VPS33A Antibody [NBP2-20872] -
Western Blot: VPS33A Antibody [NBP2-20872] - VPS33A was the direct target of miR-423-5p. (A) The location of the two miR-423-5p binding domains. Interaction domains are indicated by red font. Mutant sequences are indicated by bold font. (B) The structure diagram of the constructed plasmids. (C) A 2 × 4 (factor of miRNA: NC mimic & miR-423-5p mimic, 100 nM; factor of luciferase reporter plasmid: pmirGLO vector (mock), pmirGLO-WT, pmirGLO-MT1, pmirGLO-MT2 & pmirGLO-MT (1 + 2)) orthogonal experiment for Dual-Glo-Luciferase-Assay was performed. (D) The co-localization of LC3-GFP puncta with lysosomes in the macrophages transfected with VPS33A specific siRNA (negative siRNA as NC) was analyzed. More than 20 cells from three independent experiments were analyzed. Scale bars: 5 μm. (E) The expression levels of VPS33A in the macrophages transfected with VPS33A specific siRNA or NC siRNA were analyzed. (F) The co-localization of LC3-GFP puncta with lysosomes in the macrophages transfected with NC mimic, miR-423-5p mimic, or miR-423-5p mimic + pcDNA-VPS33A were analyzed. More than 20 cells from three independent experiments were analyzed. Scale bars: 5 μm. (G) The protein levels of VPS33A in the macrophages transfected with NC mimic, miR-423-5p mimic, or miR-423-5p mimic + pcDNA-VPS33A were analyzed by western blot. The statistical results were obtained from more than three independent experiments. Target genes were normalized with beta-actin. Data are presented as mean ± SD, & Error bars indicate the SD. NC: negative control. *P < 0.05, ** P < 0.01, *** P < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30898038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for VPS33A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VPS33A
Alternate Names
FLJ22395, FLJ23187, hVPS33A, vacuolar protein sorting 33 homolog A (S. cerevisiae), vacuolar protein sorting 33A, vacuolar protein sorting 33A (yeast), vacuolar protein sorting-associated protein 33A
Gene Symbol
VPS33A
UniProt
Additional VPS33A Products
Product Documents for VPS33A Antibody - BSA Free
Product Specific Notices for VPS33A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.