VEGF Antibody - BSA Free

Western Blot: VEGF AntibodyBSA Free [NB100-2381]

Western Blot: VEGF Antibody [NB100-2381] - Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate using.

Western Blot: VEGF Antibody - BSA Free [NB100-2381] -

HIF-1 alpha protein and HIF-1 alpha target genes are elevated in SCs following retrovirus administration. Expression of mRNA for VP16, HIF-1 alpha, and beta-actin in transfected SCs was confirmed by PCR in VP16 and VP16-HIF SCs (A). Protein expression of VP16, HIF-1 alpha, HIF-2 alpha, VEGF, enolase, and protein loading controls (GFP or beta-actin) in transfected SCs was assessed by western blotting (B). Protein expression was normalized to the loading control. Relative intensity of VP16 expression is shown in C, and the fold change in protein expression relative to control SCs is shown for HIF-1 alpha (D) HIF-2 alpha (E), VEGF (F), and enolase (G). Values are as follows: VP16 (control: 0.0 +/- 0.0; VP16: 0.40 +/- 0.01; VP16-HIF: 0.41 +/- 0.00); HIF-1 alpha (control: 1.0 +/- 0.04; VP16: 1.0 +/- 0.04; VP16-HIF: 5.9 +/- 0.01); HIF-2 alpha (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.01; VP16-HIF: 1.0 +/- 0.02); VEGF (control: 1.0 +/- 0.01; VP16: 1.02 +/- 0.02; VP16-HIF: 2.2 +/- 0.01); enolase (control: 1.0 +/- 0.01; VP16: 1.0 +/- 0.04; VP16-HIF: 1.7 +/- 0.00); n = 3/group. Mean +/- SEM, *p < 0.0001. Figure Contributions: Veena Kandaswamy generated the VP16-HIF cells. Veena Kandaswamy performed the PCRs. Ying Dai performed the western blottings. Ying Dai and Caitlin Hill analyzed the data. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31488552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.