VAP-A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83132
![Western Blot: VAP-A Antibody [NBP1-83132]](https://resources.bio-techne.com/images/products/VAP-A-Antibody-Western-Blot-NBP1-83132-img0010.jpg "Western Blot: VAP-A Antibody [NBP1-83132]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NDKLGITPPGNAPTVTSMSSINNTVATPASYHTKDDPRGLSVLKQEKQKNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASF
Reactivity Notes
Rat (84%).
Localization
Endoplasmic Reticulum
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit VAP-A Antibody - BSA Free (NBP1-83132) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VAP-A Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132]](https://resources.bio-techne.com/images/products/VAP-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83132-img0008.jpg "Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132]")
Immunocytochemistry/ Immunofluorescence: VAP-A Antibody [NBP1-83132]
Immunocytochemistry/Immunofluorescence: VAP-A Antibody [NBP1-83132] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.![Western Blot: VAP-A Antibody [NBP1-83132]](https://resources.bio-techne.com/images/products/VAP-A-Antibody-Western-Blot-NBP1-83132-img0006.jpg "Western Blot: VAP-A Antibody [NBP1-83132]")
Western Blot: VAP-A Antibody [NBP1-83132]
Western Blot: VAP-A Antibody [NBP1-83132] - Analysis in human cell line MOLT-4.
Applications for VAP-A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 5 using NBP1-83132 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VAP-A
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein A
Alternate Names
VAMP-A, VAP-33, VAPA
Entrez Gene IDs
9218 (Human)
Gene Symbol
VAPA
UniProt
Additional VAP-A Products
Product Documents for VAP-A Antibody - BSA Free
Product Specific Notices for VAP-A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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