VAChT/SLC18A3 Antibody (HL3053)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48765

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48765
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3053

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VAChT/SLC18A3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of mouse VAChT/SLC18A3. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit VAChT/SLC18A3 Antibody (HL3053) (NBP3-48765) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for VAChT/SLC18A3 Antibody (HL3053)

VAChT/SLC18A3 Antibody (HL3053)

Western Blot: VAChT/SLC18A3 Antibody (HL3053) [NBP3-48765] -

Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VAChT/SLC18A3 antibody [HL3053] (NBP3-48765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
VAChT/SLC18A3 Antibody (HL3053)

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody (HL3053) [NBP3-48765] -

VAChT/SLC18A3 antibody [HL3053] detects VAChT/SLC18A3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VAChT/SLC18A3 stained by VAChT/SLC18A3 antibody [HL3053] (NBP3-48765) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for VAChT/SLC18A3 Antibody (HL3053)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAChT/SLC18A3

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Gene Symbol

SLC18A3

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody (HL3053)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VAChT/SLC18A3 Antibody (HL3053)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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