V5 Epitope Tag Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-381B
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Validated:
Immunohistochemistry-Paraffin, Immunoblotting, ELISA
Cited:
ELISA
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 29017057).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for V5 Epitope Tag Antibody [Biotin]
Western Blot: V5 Epitope Tag Antibody [Biotin] [NB600-381B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 10 seconds.
Applications for V5 Epitope Tag Antibody [Biotin]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoblotting
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB600-381. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.2% BSA
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: V5 Epitope Tag
Alternate Names
GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5
Additional V5 Epitope Tag Products
Product Documents for V5 Epitope Tag Antibody [Biotin]
Product Specific Notices for V5 Epitope Tag Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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