USP53 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00054532-B01P
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
USP53 (AAH17382.1, 1 a.a. - 188 a.a.) full-length human protein. MDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS
Specificity
USP53 - ubiquitin specific peptidase 53,
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for USP53 Antibody - Azide and BSA Free
![Western Blot: USP53 Antibody [H00054532-B01P]](https://resources.bio-techne.com/images/products/USP53-Antibody-Western-Blot-H00054532-B01P-img0004.jpg "Western Blot: USP53 Antibody [H00054532-B01P]")
Western Blot: USP53 Antibody [H00054532-B01P]
Western Blot: USP53 Antibody [H00054532-B01P] - Analysis of USP53 expression in transfected 293T cell line by USP53 polyclonal antibody. Lane 1: USP53 transfected lysate(20.68 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: USP53 Antibody [H00054532-B01P]](https://resources.bio-techne.com/images/products/USP53-Antibody-Immunocytochemistry-Immunofluorescence-H00054532-B01P-img0003.jpg "Immunocytochemistry/ Immunofluorescence: USP53 Antibody [H00054532-B01P]")
Immunocytochemistry/ Immunofluorescence: USP53 Antibody [H00054532-B01P]
Immunocytochemistry/Immunofluorescence: USP53 Antibody [H00054532-B01P] - Analysis of purified antibody to USP53 on HepG2 cell. (antibody concentration 10 ug/ml)
Western Blot: USP53 Antibody - Azide and BSA Free [H00054532-B01P] -
Effect of EIF4A3 on USP53 expression. (A) The ENCORI websites analyzed the interaction of USP53 and EIF4A3. (B) The interaction between USP53 and EIF4A3 was detected by Co-IP. (C) BMSCs were transfected with sh-NC/sh-EIF4A3/pcDNA/EIF4A3 overexpression vector, and WB analysis was used to determine EIF4A3 protein level. (D, E) Act D assay was performed to measure the stability of USP53 mRNA. (F) WB analysis for the detection of USP53 protein expression. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40481141), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for USP53 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: USP53
Long Name
Inactive ubiquitin carboxyl-terminal hydrolase 53
Alternate Names
KIAA1350
Entrez Gene IDs
54532 (Human)
Gene Symbol
USP53
UniProt
Additional USP53 Products
Product Documents for USP53 Antibody - Azide and BSA Free
Product Specific Notices for USP53 Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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