USP47 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85942
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (93%), Rat (97%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for USP47 Antibody - BSA Free
![USP47 Antibody - BSA Free Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]](https://resources.bio-techne.com/images/products/nbp1-85942_rabbit-polyclonal-usp47-antibody-23420251592590.jpg "Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]")
Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]
Staining of human liver shows strong cytoplasmic positivity in hepatocytes.![USP47 Antibody - BSA Free Western Blot: USP47 Antibody - BSA Free [NBP1-85942]](https://resources.bio-techne.com/images/products/nbp1-85942_rabbit-polyclonal-usp47-antibody-23420251562064.jpg "Western Blot: USP47 Antibody - BSA Free [NBP1-85942]")
Western Blot: USP47 Antibody - BSA Free [NBP1-85942]
Analysis in human cell line K562.
Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -
The impact of calcification with knockdown of USP47 proteins in RASMCs. (a) The level of calcification according to real-time PCR in the control group (CTRL), the negative control group (NC), and the USP47 siRNA-transfected group (USP47 siRNA) with or without calcification (CAL). One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (b) The expression levels of USP47, RUNX2, RANKL and SM22 alpha in USP47 siRNA RASMCs according to immunoblotting. (c) Von kossa staining of USP47 siRNA RASMCs cultured in a high phosphorus environment for 72 h. The scale bar represents 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for USP47 Antibody - BSA Free
Application
Recommended Usage
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: USP47
Long Name
Ubiquitin Specific Protease 47
Alternate Names
TRFP
Gene Symbol
USP47
Additional USP47 Products
Product Documents for USP47 Antibody - BSA Free
Product Specific Notices for USP47 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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